Storage protocol of dental pulp cells from human exfoliated deciduous teeth.
نویسندگان
چکیده
منابع مشابه
isolation of mesenchymal stem cells from dental pulp of exfoliated human deciduous teeth
objective: the exfoliated human deciduous tooth (shed) contain multipotent stem cells that identified to be a population of highly proliferative and clonogenic .these cells are capable of differentiating into a variety of cell types including neural cells, adipocytes, and odontoblasts. material and methods: normal exfoliated human deciduous incisors collected from six- to nine-years-old childre...
متن کاملThe Comparison of the Immunologic Properties of Stem Cells Isolated from Human Exfoliated Deciduous Teeth, Dental Pulp, and Dental Follicles
Aim. To compare the effects of various mesenchymal stem cells, those isolated from human exfoliated deciduous teeth (SHEDs), dental pulp stem cells (DPSCs), and dental follicle stem cells (DFSCs), on human peripheral blood mononuclear cells (PBMCs). Method. Mesenchymal stem cells were isolated from three sources in the orofacial region. Characterization and PCR analyses were performed. Lymphocy...
متن کاملSHED: stem cells from human exfoliated deciduous teeth.
To isolate high-quality human postnatal stem cells from accessible resources is an important goal for stem-cell research. In this study we found that exfoliated human deciduous tooth contains multipotent stem cells [stem cells from human exfoliated deciduous teeth (SHED)]. SHED were identified to be a population of highly proliferative, clonogenic cells capable of differentiating into a variety...
متن کاملIsolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp
This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC) isolation from human deciduous teeth pulp. A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital. The MSCs were isolated from dental pulps using a standard protocol. In total, 128 colonies of MSCs were obtained and the success rate was 79.5%. Compared to teeth not ...
متن کاملThe Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth
STATEMENT OF THE PROBLEM Stem cells from human exfoliated deciduous teeth (SHEDs) are a population of highly proliferative cells, being capable of differentiating into osteogenic, odontogenic, adipocytes, and neural cells. Vitamin D3 metabolites such as 1α, 25-dihydroxyvitamin D3 are key factors in the regulation of bone metabolism. PURPOSE The aim of this study was to investigate the effect ...
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ژورنال
عنوان ژورنال: Brazilian Dental Science
سال: 2017
ISSN: 2178-6011
DOI: 10.14295/bds.2017.v20i3.1457